Description
| Catalogue Number | P01240 | ||||||||||||||||
| Description | Bi-modal Ultra Small Particle of Iron Oxyde (USPIO) Guerbet Research Prototype designed for macrophage imaging. This compound is equivalent to P904 but contains also Rhodamine B for a fluorescent detection | ||||||||||||||||
| Main Applications | Cellular imaging: inflammation through macrophage imaging after i.v. injection (atherosclerotic plaque, Alzheimer disease, multiple sclerosis, osteo-arthritis…), metastatic lymph node imaging, liver tumor, in vitro cell labeling and cell trafficking | ||||||||||||||||
| Molecular characteristics |
– Hydrodynamic size: diameter is 25-30 nm
– Fe content : > 5000 Fe atoms per particle
– Fluorescent content: 7 rhodamines per particle
– Recommended dose: 200 to 1000 μmol/kg = 2.5 μL/g
Example: for a 30g body weight mouse injection, volume is 75 μL
– Relaxivities ( s-1.mM-1, 37°C; 4% Human Albumin Serum, medium closed to plasma)
– Qualitative techniques: Perl’s staining
– In vitro quantification techniques: P01240 is a Fe-based contrast agent. Thus, it is necessary to take into account the endoge- nous iron content for quantification in biological media by ICP- AES or ICP-MS. Another way for USPIO quantification is rela- xometry, which is poorly sensitive to endogenous iron content.
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| Characteristics of the vial | – Concentration: [Fe] = 4.47 mgFe/L
– Volume: 450 μL (5 mice dose approx.) – Other animal species: contact us |
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| Storage | Need to be stored at 4°C in a closed vial away from light | ||||||||||||||||
| Notes | For in vitro or animal use only | ||||||||||||||||
| Selected publications | [1] Corot et al, ed. Molecular and Cellular MR Imaging: CRC Press, 2007 [2] Unpublished Guerbet data [3] Sigovan et al, Radiology 2009 [4] Raynaud et al, ISMRM 2010 [5] Luciani et al, Radiology 2012 | ||||||||||||||||
